NCT02887547

Biological Markers Evaluation of Accelerated Tooth Movement

Biomarkers Expression in Gingival Crevicular Fluid and Accelerated Tooth Movement

• Status: Unknown
• Phase: N/A
• Study type: Interventional
• Enrollment: 25 (actual)
• Sponsor: University of Sao Paulo · Academic / Other
• Sex: All
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Gingival Crevicular Fluid samples will be collected using periopapers after accelerated tooth movement technique, results will be assessed with biomarkers levels done using ELISA Assays

Detailed description

For this prospective study, 22 individuals were selected, with skeletal discrepancies randomly divided into two groups: 1 - accelerated tooth movement technique (ATMT) and 2 - Control. Orthodontic records done in two times, initial and preoperative; Gingival Crevicular Fluid samples will be collected using periopapers in 7 times (1, 2, 7, 14, 28 days and after space closure- end of orthodontic treatment movement activation). Flapless cortical perforations will be made in ATMT Group after dental extraction. Surgical protocol will be flapless to preserve periodontal health. After extraction space closure, results will be assessed by clinical effects with biological markers levels: CCL3/MIP-1 alpha, CCL5/RANTES, CXCL8/IL8, IL-1 alpha, IL-1 beta, IL6, TNF-alpha, TRANCE/RANK L, OCN OPG IL-10 VEGF TGF-ℬ Those biomarkers levels will be performed using ELISA Assays. After collecting data, the results will be statistically analyzed using Shapiro-Wilk and, posteriorly, T-student or Wilcoxon.

Conditions

• Cytokine Measurements
• Orthodontic Space Closure
• Tooth Movement

Interventions

Type	Name	Description
PROCEDURE	micro-osteoperforation

Timeline

• Start date: 2016-08-01
• Primary completion: 2018-02-01
• Completion: 2020-05-01
• First posted: 2016-09-02
• Last updated: 2019-07-01