NCT02853968

Unlock the Cell: Castleman's Disease Flow Cytometry Study

Unlock the Cell: Intracellular Inflammatory Pathways and Flow Cytometry Study for Castleman's Disease

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 130 (actual)
• Sponsor: University of Pennsylvania · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Accepted

Summary

Castleman disease, a rare lymphoproliferative disorder, is characterized by inflammatory cytokine production and multiple organ system dysfunction. In this study, we will investigate inflammatory markers, cells, and signaling pathways in prospectively collected blood samples and/or buccal swabs or saliva using biochemical and RT-PCR techniques, proteomics, genomics, immunohistochemistry, storage for future use, cell culture treated with external stimuli, flow cytometry, and other molecular tests

Detailed description

This is a University of Pennsylvania-sponsored project that is supported by the Castleman Disease Collaborative Network and the patients/loved one's group Castleman's Warriors (Castleman's Awareness and Research Effort). Castleman Disease (CD) is a rare and poorly understood lymphoproliferative disease. The multicentric CD subtype (MCD) involves enlarged lymph nodes in multiple regions of the body and can be fatal if untreated. MCD patients demonstrate acute inflammatory crisis due to upregulation of inflammatory agents most notably IL-6 and VEGF followed by multiple organ failure and death. Unlock the Cell aims to identify the pathways the disease takes through flow cytometry studies. The purpose of the CD Research study is to collect blood samples and/or buccal swabs or saliva samples and medical information of MCD patients and compare them to control samples so researchers can understand the causes of MCD, and design treatments based on our findings. In this study, the investigators will analyze inflammatory markers, cells, and signaling pathways in prospectively collected blood samples using biochemical and RT-PCR techniques, proteomics, genomics, immunohistochemistry, storage for future use, cell culture treated with external stimuli, flow cytometry, and other molecular tests. A secondary aim is to collect excess stored tissue samples (e.g., lymph node, bone marrow) from previous procedures and store these samples along with unused blood samples for future research purposes to be performed at the University of Pennsylvania or shared with other Castleman disease researchers and biobanks.

Conditions

• Castleman Disease
• Angiofollicular Lymphoid Hyperplasia
• GLNH
• Hyperplasia
• Castleman's Disease
• Angiofollicular Lymph Hyperplasia
• Giant Lymph Node Hyperplasia

Interventions

Type	Name	Description
PROCEDURE	Blood draw/buccal swab	The research project will need a blood sample of no more than 50mL per two month period. The research project may also request a buccal swab from patients if needed.

Timeline

• Start date: 2016-02-01
• Primary completion: 2019-01-30
• Completion: 2019-03-30
• First posted: 2016-08-03
• Last updated: 2020-03-26

Locations

1 site across 1 country: United States