NCT02660723

Impact of Aging on Cytokine Production by Innate Immune Cells

To Investigate the Impact of Aging on Cytokine and Chemokine Production by Peripheral Blood Immune Cells

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 50 (actual)
• Sponsor: Centre Hospitalier Universitaire de Nice · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

The main goal of this study is to investigate the impact of aging on the responsiveness of peripheral blood immune cells. To this aim, the investigators will take advantage of a recently described syringe-based assay system that can reproducibly assess induced, innate or adaptive immune responses. Briefly, a total of 50 healthy volunteers will participate to this study: 25 will be older than 18 and younger than 30, and 25 will be older than 55. A total of 14 ml of blood will be drawn including 5 X 1 ml in TruCulture™ tubes containing poly(I:C), R-848, LPS, CpG, or no stimulus, respectively. After 22 hours of incubation at 37°C, cellular supernatants will assessed for levels of 10 different chemokines using a MesoScaleDiscovery multiplexing platform.

Conditions

• Healthy

Interventions

Type	Name	Description
OTHER	Healthy Volunteers	A catheter will be introduced in the arm vein and 14 ml of blood will be drawn in one 4 ml dry tube, one 5 ml heparinized tube for cell count and 5 1 ml TruCulture tubes.

Timeline

• Start date: 2016-02-01
• Primary completion: 2017-02-01
• Completion: 2018-06-01
• First posted: 2016-01-21
• Last updated: 2018-07-23

Locations

1 site across 1 country: France