NCT02560818

Transcriptomes Breast, Ovarian and Leukocyte Hereditary Genes Predisposing to Breast and / or Ovarian Cancer

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 20 (actual)
• Sponsor: Centre Francois Baclesse · Academic / Other
• Sex: Female
• Age: 18 Years – 65 Years
• Healthy volunteers: Accepted

Summary

This is a case-control study of molecular diagnostics. This study requires two steps: * The first part of the study will be conducted on a population of 20 women without breast cancer and, or ovarian family (Healthy Volunteers controls) * The second part of the study will be conducted on a population of 50 patients predisposed to familial breast and, or ovarian cancer compared to 20 controls . For analysis of leucocytes of the patients, a blood sample collected in a prior study (EXSAL study, ID-RCB 2009-A00833-54) will be used.

Detailed description

This study requires two steps: \- The first part of the study will be conducted on a population of 20 women free of breast cancer and, or ovarian Family (healthy volunteers controls) and without a known family history of breast and or ovarian cancer. Mapping the level of blood (leukocytes) and the breast tissue will be drawn for the same individual on the physiological profiles splicing of the messenger RNA of genes involved in this predisposition, targeted by high-throughput sequencing of RNA (RNASeq). A direct comparison of RNA splicing patterns between blood and breast tissue from the same witness will detect any differences between these two tissues. -The second part of the study will be conducted on a population of 50 patients predisposed to familial breast and, or ovarian cancer compared to 20 controls with no known family history of breast and, or ovarian cancer. Analysis of the results will highlight potential splicing abnormalities.

Conditions

• Breast Cancer
• Transcriptomes
• Molecular Diagnostic
• Ovarian Cancer

Interventions

Type	Name	Description
OTHER	collection of a blood sample and breast and ovarian tissue	a blood sample and a breast and ovarian tissue sample will be collected in healthy women during their surgery (breast or ovarian surgery according to selection group :A, B or C)
OTHER	use of a previous blood collection for patients

Timeline

• Start date: 2015-10-01
• Primary completion: 2020-10-01
• Completion: 2020-10-01
• First posted: 2015-09-25
• Last updated: 2020-11-09

Locations

1 site across 1 country: France