NCT02425800

Human Prostate Tissue Model to Maintain and Study Prostate Cancer Stem Cells

Optimizing Human Prostate Extracellular Matrix as a Structure for Maintenance and Studying of Prostate Cancer Stem Cells

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 43 (actual)
• Sponsor: Wake Forest University Health Sciences · Academic / Other
• Sex: Male
• Age: —
• Healthy volunteers: Not accepted

Summary

This pilot research trial studies the use of a human prostate tissue model to maintain and study prostate cancer stem cells. A human prostate tissue model uses leftover tissue that was removed during surgery from patients with non-cancerous enlargement of the prostate (benign prostatic hyperplasia) and may create an environment similar to the natural environment of the human body. Prostate cancer stem cells are cells that cause cancer to grow. Using real tissue to create an environment to study stem cells may help doctors learn more about how they work and how they respond to treatments.

Detailed description

PRIMARY OBJECTIVES: I. To optimize a decellularized prostate tissue model for the maintenance of prostate cancer stem cells. SECONDARY OBJECTIVES: I. To investigate the self-renewal and differentiation ability of human prostate cancer stem cells (CSCs) (tumor-associated calcium signal transducer 2 \[TROP2\]+ cells) in the above mentioned decellularized prostate tissue model. II. To compare the number of CSCs according to key patient characteristics, including race, age, Gleason, metastasis status, and previous cancer treatment(s). OUTLINE: Tissue samples are collected from patients with benign prostatic hyperplasia for decellularization and preparation as human extracellular matrix for growing human prostate CSCs. Tissue samples are also collected from patients with prostate cancer for the analysis of TROP2+ cells by flow cytometry.

Conditions

• Benign Prostatic Hyperplasia
• Prostate Carcinoma

Interventions

Type	Name	Description
OTHER	Cytology Specimen Collection Procedure	Undergo collection of tissue samples
OTHER	Laboratory Biomarker Analysis	Correlative studies

Timeline

• Start date: 2015-07-28
• Primary completion: 2018-11-05
• Completion: 2021-08-03
• First posted: 2015-04-24
• Last updated: 2021-09-01

Locations

2 sites across 1 country: United States