NCT02420886

Adding Cytokines to In Vitro Human Culture Media to Improve Embryogenesis and Implantation

The Effects of GM-CSF, HB-EGF and LIF Cytokines Supplementation Need Verification for Human Embryo Development in Vitro

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 443 (actual)
• Sponsor: Ibn Sina Hospital · Other Government
• Sex: Female
• Age: 18 Years – 40 Years
• Healthy volunteers: Accepted

Summary

Assessment of the embryological benefit of in vitro adding of Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2n/ml) to the culture media and closely monitor its effect on embryogenesis and implantation outcomes.

Detailed description

The IVF culture media design and upgrade underwent many ideas during the last 3 decades, we suppose that adding the Cytokines (LIF 5ng/ml, HB-EGF 5ng/ml and GM-CSF 2ng/ml) as a growth factor that have multi-ways to might improve embryo development in vitro so we decide to design a trial to compare the embryogenesis by sibling Oocytes of the same patient between two arm of the same culture media and the same culture condition. One arm with Cytokines and the other is traditional one and closely monitor the outcome within the 2 arms and record the results.

Conditions

• Infertility

Interventions

Type	Name	Description
OTHER	Adding LIF, HB-EGF and GM-CSF to Lifeglobal human IVF culture media

Timeline

• Start date: 2015-05-16
• Primary completion: 2016-03-01
• Completion: 2016-03-01
• First posted: 2015-04-20
• Last updated: 2018-10-02

Locations

2 sites across 1 country: Egypt