NCT02328534

Semen Processing for ICSI Using Swim-down and Density Gradient Methods

Novel Approach of Semen Preparation to Improve ICSI Outcome.

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 890 (actual)
• Sponsor: Ahmad Mustafa Mohamed Metwalley · Academic / Other
• Sex: All
• Age: 20 Years – 38 Years
• Healthy volunteers: Not accepted

Summary

The investigators evaluate the feedback of using swim down semen processing method during preparation for ICSI and compare the results of pregnancy and embryos quality with the cases used density gradient method for their semen processing.

Detailed description

There is different semen processing method, in this study we compared the ICSI output when the investigators used one method with higher separation method and minimum sperm stress or physical interface as swimming down using 100 % silica solution, with other more stressful procedure with high selection power as density gradient. The routine used method as density gradient (DG) includes about 30 min centrifugation at 1200 RPM, and more working steps with more handling procedures. According to many references the investigators can approve that it is method to be indicated with all cases particularly with those severely decreases semen number or motility. Using 100 % silica solution for semen processing used for ICSi, shews lesser centrifugation time and minimum processing procedures. As swimming down time is about 30 min followed by 5 min washing and finalized with other 3 minutes last wash. For that total interfering physical action is about 8 minutes compared with 30 min with DG method. In swimming down we are using the natural sperm behavior and own swimming power for separate itself from other seminal components. As well as only the one normally structure and motility direction the only can penetrate the interface surface of silica solution and swim inside higher density matrix using its speed and power.

Conditions

• Infertility

Interventions

Type	Name	Description
BEHAVIORAL	Blastocyst Formation Rate	Embryos developed at Day3 cultures, are continued for Day5 culture tho reach blastocyst stage. The percentage of blastocyst produced from cleaved embryos to be evaluated ar rate. The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.
PROCEDURE	Blastocyst Rate	Day 5 embryo transfer achieved pregnancy are compared with total embryo transfer cases at Day5. The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time. Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam.
PROCEDURE	Pregnancy Rate	All pregnancy achieved from all ET. Pregnancy confirmed with day 12 after Et and repeated with 48 hours after 1st exam. The evaluation with doe one time using Density Gradient semen one time and Swimming down semen another time.

Timeline

• Start date: 2007-03-01
• Primary completion: 2013-09-01
• Completion: 2014-01-01
• First posted: 2014-12-31
• Last updated: 2014-12-31

Locations

2 sites across 2 countries: Bahrain, Egypt