NCT02295488

Determinism of the Mechanism of Action of Specific Immunotherapy in Hymenoptera Venom Allergy

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 29 (actual)
• Sponsor: University Hospital, Rouen · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

Currently, most of the protocols used for desensitization to Hymenoptera venoms consist of a very rapid increase in doses of venom extracts administered during the SIT in a day without systemic reaction of the patient which is not yet explained. The hypothesis is that this good tolerance is due to a rapid decrease in the response of mast cells and basophils to the allergen.. The main objective is to study basophil activation in response to the allergen during the first day of desensitization, measured by basophil activation test. The initiation of desensitization follow a protocol of rapid growth rates. The basophil activation level in the course of desensitization will be analyzed by flow cytometry after in vitro stimulation by allergen. Changes in T cell polarization is also analyzed by flow cytometry. Evolution of the expression of different proteins in blood basophils will be studied by measuring their messenger RNAs

Conditions

• Hymenoptera Allergy

Interventions

Type	Name	Description
PROCEDURE	biological sampling	blood intake for biological sampling during validated desensitization protocol (Alyostall®)
BIOLOGICAL	Desensitization protocol (Alyostall®)	Validated desensitization protocol using Alyostall® performed

Timeline

• Start date: 2015-03-23
• Primary completion: 2018-04-12
• Completion: 2018-04-12
• First posted: 2014-11-20
• Last updated: 2019-10-25

Locations

1 site across 1 country: France