NCT02040337

Sequencing T-cells in Type I Diabetes Mellitus

Sequencing T-cell Receptor Repertoire of Auto-Antigen Specific T-cells in Type I Diabetes Mellitus

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 40 (actual)
• Sponsor: Seton Healthcare Family · Academic / Other
• Sex: All
• Age: 18 Years – 55 Years
• Healthy volunteers: Accepted

Summary

The purpose of this study is to investigate CD8 + T-cell receptor (TCR) repertoire differences between patients with type 1 diabetes mellitus and type 2 diabetes or healthy controls.

Detailed description

Using a systems biology approach by combining high-throughput sequencing, cytometry, gene expression, transcriptome profiling, HLA typing, single cell analysis, and TCR affinity assays, the investigators will define a set of immune metrics for the prediction or early diagnosis of disease that is superior to the current examination of auto-antibodies, which is a fairly late stage in the disease development. This can also be used to guide therapy in advanced disease.

Conditions

• Diabetes Mellitus, Type 1
• Diabetes Mellitus, Type 2

Timeline

• Start date: 2014-04-01
• Primary completion: 2017-09-01
• Completion: 2017-12-13
• First posted: 2014-01-20
• Last updated: 2018-04-17

Locations

1 site across 1 country: United States