NCT01908569

Application of MACS and Time-lapse Technology in Good-prognosis Patients

Application of MACS (Magnetic-activated Cell Sorting) and Time-lapse Technology (Embryoscope) in Assisted Reproduction Patients With Good Prognosis

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 66 (actual)
• Sponsor: Vida Recoletas Sevilla · Academic / Other
• Sex: Female
• Age: 18 Years – 38 Years
• Healthy volunteers: Not accepted

Summary

The MACS technique (magnetic-activated cell sorting) is based on the usage of microbeads, magnetic particles with a diameter of 35 nanometers joined to the compound annexin V. Annexin V is a protein with a high affinity for the molecule phosphatidylserine. This system allow us to eliminate those spermatozoids which have begun the apoptosis and have phosphatidylserine residues on their membrane surface. Using this technique, the apoptotic spermatozoids will join the microbeads and consequently be retained in a column applying a magnetic field. Recent studies show the benefits of spermatozoid selection using MACS and the improvement in mobility, viability, DNA fragmentation and fertilization potential. It has also been observed an improvement in the embryo cells division rate, clinical pregnancy rates and spermatozoid survival after thawing. However, it is necessary to perform new clinical studies in order to evaluate the real potential of this technology and its safety. The purpose of this study is to evaluate the effect of the MACS technique on the clinical results of good-prognosis patients who are going through IVF/ICSI treatments in order to achieve a pregnancy. The hypothesis of this study is that the application of the MACS technique in assisted reproduction treatments is associated with a better embryo quality and pregnancy rates.

Conditions

• MACS Technique Efficacy

Interventions

Type	Name	Description
OTHER	MACS	The sperm will be subjected to capacitation through a density gradient together with the MACS technique in order to select the non-apoptotic spermatozoids.
OTHER	NO MACS	The sperm capacitation will be performed through a density gradient. The MACS technique will not be applied.

Timeline

• Start date: 2013-07-01
• Primary completion: 2016-11-01
• Completion: 2016-11-01
• First posted: 2013-07-25
• Last updated: 2018-09-05

Locations

1 site across 1 country: Spain