NCT01800253

The Effects of Acute Total Sleep Deprivation Versus Normal Sleep on Metabolism

The Role of Acute Total Sleep Deprivation in the Regulation of Metabolism, Neuroendocrine Responses, and Behavioral Measures

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 17 (actual)
• Sponsor: Uppsala University · Academic / Other
• Sex: Male
• Age: 18 Years – 28 Years
• Healthy volunteers: Accepted

Summary

The study proposes to investigate whether acute total deprivation affects metabolism as measured through blood and peripheral tissues. Its aim is also to investigate how acute total sleep deprivation affects neurodegenerative markers, as well as hormones, memory performance and aspects of appetite regulation.

Detailed description

It is predicted that acute total sleep deprivation will affect gene expression and DNA methylation. It is also predicted that sleep deprivation will up-regulate ghrelin, and affect other neuroendocrine markers and hormones in a negative manner. It is further predicted that sleep deprivation will decrease participants' memory performance.

Conditions

• Sleep Deprivation
• Sleep

Interventions

Type	Name	Description
BEHAVIORAL	Inhibitory task	Participants perform a binary decision on each presented stimuli. Of the two possible outcomes, participants are instructed to make a motor response (go) for one type, and are to withhold a response (no-go) for the other type. Reaction time and accuracy are measured for each event
PROCEDURE	Blood samples	Hormone levels, neuromolecular levels and gene expression profiles will be analyzed from repeated blood samples obtained before and after the nighttime intervention
PROCEDURE	Tissue samples	Expression profiles will be analyzed from samples obtained from tissues involved in metabolism
PROCEDURE	Oral glucose tolerance test	75 g of glucose will be dissolved in 300 ml of water and given to participants, followed by blood sampling at 0, 15, 30, 60, 90, 120 and 150 minutes following the ingestion of the glucose solution.
BEHAVIORAL	Portion Size Task	Participants are given a computer program that gives them the opportunity to choose the portions of a variety of food items that they would ideally like to consume

Timeline

• Start date: 2013-03-01
• Primary completion: 2013-10-01
• Completion: 2013-10-01
• First posted: 2013-02-27
• Last updated: 2013-12-09

Locations

1 site across 1 country: Sweden