NCT01594645

The Clinical Impact of Selecting Acrosome Reacted Spermatozoa for ICSI

The Impact of Selecting Acrosome Reacted Spermatozoa for ICSI- a Prospective RCT

• Status: Withdrawn
• Phase: Phase 1
• Study type: Interventional
• Enrollment: 0 (actual)
• Sponsor: Hadassah Medical Organization · Academic / Other
• Sex: All
• Age: 18 Years – 38 Years
• Healthy volunteers: Not accepted

Summary

Presently spermatozoa for ICSI are selected based on morphology and motion, without determining if acrosome reaction (AR) had occurred during sperm preparation or not. Although not critical if the sperm is injected into the ooplasm, the occurrence of AR might might be an indicator of better spermatozoon quality and implantation potential, especially in severe OTA cases. It is impossible with conventional plain optic microscopy used in IVF units to determine in vivo (without fixation) if AR has occurred, and as a result spermatozoa are injected randomly. It is readily possible to distinguish AR+ from AR- spermatozoa using polarized light microscopy due to different bifriengance. In this study the investigators seek to determine, by a prospective RCT, if selecting only acrosome reacted spermatozoa, in severe OTA cases, will improve the outcome of ICSI and the entire IVF process.

Detailed description

A prospective RCT to determine the effect of polscope based spermatozoa selection for ICSI.

Conditions

• Infertility

Interventions

Type	Name	Description
PROCEDURE	Sperm selection by conventional / polarized light microscopy	Selecting spermatozoa for ICSI using conventional and polarized light microscopy

Timeline

• Start date: 2012-09-01
• Primary completion: 2015-08-01
• Completion: 2015-08-01
• First posted: 2012-05-09
• Last updated: 2015-08-20

Locations

1 site across 1 country: Israel