NCT01576211

Studying Genes Using Cord Blood and Placenta Samples From Relatively Healthy Newborns and Samples From Younger Patients With Wilms Tumor

Investigating the Frequency of Loss of Imprinting Across a Birth Cohort and the Link DNA Methylation Plays

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 40 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 21 Years
• Healthy volunteers: Accepted

Summary

RATIONALE: Studying samples of blood and tissue from newborns and from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies cord blood and placenta tissue from newborns, and tumor tissue samples from patients with Wilms tumor.

Detailed description

OBJECTIVES: * What is the frequency of loss of imprinting at birth (in the cord blood and placenta) in a relatively healthy birth cohort? * Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct association to the gene expression? OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA methylation, single nucleotide polymorphism, and gene expression by polymerase chain reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender and age of the samples are also collected, if possible.

Conditions

• Kidney Cancer

Interventions

Type	Name	Description
GENETIC	DNA methylation analysis
GENETIC	RNA analysis
GENETIC	allele-specific oligonucleotide real-time quantitative polymerase chain reaction
GENETIC	gene expression analysis
GENETIC	nucleic acid sequencing
GENETIC	polymorphism analysis
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2012-04-01
• Primary completion: 2016-05-01
• First posted: 2012-04-12
• Last updated: 2016-05-18