NCT01558349

Circulating Tumor Cells and Melanoma: Comparing the EPISPOT and CellSearch Techniques

Circulating Tumor Cells and Melanoma: Comparing the EPISPOT (EPithelial ImmunoSPOT) and CellSearch Techniques

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 73 (actual)
• Sponsor: Centre Hospitalier Universitaire de Nīmes · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

The main objective of this study is to compare results for the detection of circulating melanoma cells (CMC) using CellSearch versus EPISPOT (EPithelial ImmunoSPOT) techniques between a group of patients with metastatic melanoma and a group of hospitalized control patients.

Detailed description

The secondary objectives of this study include: A. To compare the following elements between the two patient groups: * the number of CMCs per ml of blood as determined by EPISPOT * the number of CMCs per ml of blood as determined by CellSearch * the percentage of patients with at least 2 CMCs per ml of blood according to the two techniques * the % of CMCs expressing KI67 * the % of CMCs expressions S100 (only the EPISPOT technique) B. To compare the EPISPOT and CellSearch techniques is terms of the following: * the number of CMCs detected per ml blood * the number of CMCs expressing antigen KI67 C. To re-evaluate the 2-CMC per ml blood threshold

Conditions

• Metastatic Melanoma

Timeline

• Start date: 2013-06-27
• Primary completion: 2017-06-27
• Completion: 2017-06-27
• First posted: 2012-03-20
• Last updated: 2025-11-19

Locations

2 sites across 1 country: France