NCT01553448

Studying Gene Expression in Samples From Younger Patients With Neuroblastoma

The Role of Stroma-Derived Soluble TßRIII in Neuroblastoma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 75 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 21 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of blood and tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors find better ways to treat cancer. PURPOSE: This research trial studies gene expression in samples from younger patients with neuroblastoma.

Detailed description

OBJECTIVES: * Determine whether TβRIII expression and TGF-β signaling decrease in advanced-stage neuroblastoma (NBL) and whether these changes are confined to the Schwannian stroma. * Determine whether sTβRIII levels and TGF-β signaling correlate with NBL stage, tumor stroma content, surface TβRIII expression, and TGF-β signaling. OUTLINE: Archived paraffin-embedded tissue and plasma samples are analyzed for TβRIII expression, TGF-β signaling, and SMAD3 expression and phosphorylation by immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and other assays. Surface expression of TβRIII in the neuroblastic and stromal tumor components are correlated with matched circulating levels of soluble TβRIII. Results are then correlated with each patient's outcome data, including stage.

Conditions

• Neoplastic Syndrome
• Neuroblastoma

Interventions

Type	Name	Description
GENETIC	gene expression analysis
GENETIC	protein expression analysis
OTHER	enzyme-linked immunosorbent assay
OTHER	immunohistochemistry staining method
OTHER	laboratory biomarker analysis
OTHER	medical chart review

Timeline

• Start date: 2012-03-01
• Primary completion: 2016-05-01
• First posted: 2012-03-14
• Last updated: 2016-05-18