Trials / Completed
CompletedNCT01542333
Studying Biomarkers in Samples From Younger Patients With Wilms Tumor
Biological Analysis of Ethnic Variations in Wilms Tumor
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 20 (estimated)
- Sponsor
- Children's Oncology Group · Network
- Sex
- All
- Age
- —
- Healthy volunteers
- Not accepted
Summary
RATIONALE: Studying samples of tissue and urine from patients with cancer in the laboratory may help doctor learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in samples from younger patients with Wilms tumor.
Detailed description
OBJECTIVES: * To determine a unique molecular "finger print" of primary Wilms tumor arising in ethnically diverse children who are at increased risk to develop this lethal malignancy and subsequently relapse. * To determine a unique protein "finger print" from the urine of ethnically diverse children who develop Wilms tumor and subsequent relapse. OUTLINE: Formalin-fixed paraffin-embedded and snap-frozen primary tissue samples, and urine samples collected at the time of diagnosis and before treatment, are analyzed for molecular and protein profile by MALDI-TOF mass spectroscopy, western blotting, immunohistochemistry, and reverse-transcriptase polymerase chain reaction (RT-PCR) assays.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| GENETIC | gene expression analysis | |
| GENETIC | proteomic profiling | |
| GENETIC | reverse transcriptase-polymerase chain reaction | |
| GENETIC | western blotting | |
| OTHER | immunohistochemistry staining method | |
| OTHER | laboratory biomarker analysis | |
| PROCEDURE | spectroscopy |
Timeline
- Start date
- 2012-02-01
- Primary completion
- 2016-05-01
- First posted
- 2012-03-02
- Last updated
- 2016-05-18
Source: ClinicalTrials.gov record NCT01542333. Inclusion in this directory is not an endorsement.