NCT01531907

Relationships Between GLP-2 and Markers of Bone Turnover Following Feeding

Relationships Between GLP-2 and Markers of Bone Turnover Following Feeding: the Influence of Obesity and Cellular Mechanisms of Action

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 19 (actual)
• Sponsor: Sheffield Teaching Hospitals NHS Foundation Trust · Academic / Other
• Sex: Female
• Age: 25 Years – 40 Years
• Healthy volunteers: Accepted

Summary

This study has been designed to study differences bone turnover in relation to glucagon-like peptide-2 (GLP-2) following feeding in lean and obese premenopausal women. Given the preliminary evidence that GLP-2 may act directly on osteoclasts, the investigators plan to determine whether GLP2 receptors are expressed in osteoclasts and the effect of GLP-2 on bone resorption. Hypotheses: 1. Acute responses of GLP-2 and bone resorption markers following feeding are affected by body fat mass. 2. Serum levels of GLP-2 are lower in obese pre-menopausal women and are associated with a reduction in trabecular and/or cortical bone mass 3. GLP-2 has direct actions on osteoclast resorption via a functional receptor

Detailed description

Study objectives: 1. To determine if differences in baseline serum levels of GLP-2 are related to differences in bone microarchitecture, structure and strength at the distal tibia and distal radius between obese and lean premenopausal females. 2. To determine whether obesity in premenopausal females influences levels of circulating GLP-2 following a standardised glucose meal with a resultant change in markers of bone formation and resorption 3. To test whether GLP-2 directly affects osteoclast function via an identifiable receptor

Conditions

• Obesity

Interventions

Type	Name	Description
OTHER	Study visit procedures	Anthropometric measurements to include: * Weight, height, and BMI. * Waist and hip circumference * Triceps skinfold thickness * Measurement of supine abdominal thickness A baseline blood sample (15ml)is taken before receiving a standard polycal drink (75g glucose load) Three further blood samples (15 ml) will be taken, at the time points 20, 60 and 120 minutes after glucose loading. In 10 subjects (5 lean and 5 obese) we will take an additional blood sample (up to 50 ml) at baseline into heparin to grow osteoclasts in culture. Visit duration - approximately 3-4 hours.

Timeline

• Start date: 2011-09-01
• Primary completion: 2014-07-01
• Completion: 2014-07-01
• First posted: 2012-02-13
• Last updated: 2019-02-15

Locations

1 site across 1 country: United Kingdom