NCT01510600

Studying Biomarkers in Samples From Patients With High-Risk Neuroblastoma

Alternative Lengthening of Telomeres (ALT) in Neuroblastoma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 99 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 30 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of blood and tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in samples from patients with high-risk neuroblastoma.

Detailed description

OBJECTIVES: * To establish telomere length measurement by quantitative polymerase chain reaction (qPCR) as an alternative lengthening of telomeres (ALT) detection method in neuroblastoma (NB). * To determine the frequency of ALT in high-risk NB and the characteristics of ALT+ NB. * To establish C-circle (extra-chromosomal telomeric DNA circles) level as a marker of ALT activity in NB. * To evaluate the prognostic significance of ALT in NB. * To evaluate the utility of the C-circle assay for the detection of circulating tumor DNA in NB patients with an ALT+ tumor. OUTLINE: Archived tumor tissue and serum samples are analyzed for telomere length measurement, frequency, and C-circle levels by PCR. Results are then compared with patients' age at diagnosis and outcomes including survival data (event-free and overall survival).

Conditions

• Neuroblastoma

Interventions

Type	Name	Description
GENETIC	DNA analysis
GENETIC	polymerase chain reaction
OTHER	laboratory biomarker analysis
OTHER	medical chart review

Timeline

• Start date: 2012-01-01
• Primary completion: 2016-05-01
• Completion: 2016-05-01
• First posted: 2012-01-16
• Last updated: 2016-05-19