Trials / Completed
CompletedNCT01490801
Biomarkers in Blood and Tissue Samples From Patients With Epstein-Barr Virus-Positive Hodgkin Lymphoma
Role of the IL-2 Inducible Tcell Kinase in EBV-HLH and EBV+ Hodgkin's Lymphoma
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 50 (estimated)
- Sponsor
- Children's Oncology Group · Network
- Sex
- All
- Age
- 9 Years
- Healthy volunteers
- Not accepted
Summary
RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in blood and tissue samples from patients with Epstein-Barr virus positive Hodgkin lymphoma.
Detailed description
OBJECTIVES: * Evaluate germline DNA from patients with Epstein-Barr virus positive (EBV+) and Hodgkin lymphoma (HL) for inducible T-cell kinase (ITK) mutations. * Examine the effects of ITK mutations on expression of the ITK protein. * Determine whether ITK mutations correlate with specific clinical or histopathological features of HL. OUTLINE: Archived blood and tumor tissue samples are analyzed for germline DNA expression and inducible T-cell kinase (ITK) mutations by PCR, IHC, flow cytometry, and western blotting, and EBV-encoded RNA (EBER) using in situ hybridization. Each patient's data, such as date, sex, age, tumor stage and histology at diagnosis, treatment received, response to treatment, development of recurrent disease, date of last follow-up, and outcomes are also collected.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| GENETIC | DNA analysis | |
| GENETIC | RNA analysis | |
| GENETIC | cytogenetic analysis | |
| GENETIC | in situ hybridization | |
| GENETIC | mutation analysis | |
| GENETIC | polymerase chain reaction | |
| GENETIC | western blotting | |
| OTHER | flow cytometry | |
| OTHER | immunohistochemistry staining method | |
| OTHER | medical chart review |
Timeline
- Start date
- 2011-12-01
- Primary completion
- 2016-05-01
- Completion
- 2016-05-01
- First posted
- 2011-12-13
- Last updated
- 2016-05-17
Source: ClinicalTrials.gov record NCT01490801. Inclusion in this directory is not an endorsement.