Trials / Completed
CompletedNCT01480518
Biomarkers in Tissue Samples From Patients With Ewing Sarcoma
Analysis of GGAA-Microsatellites in Ewing Sarcoma
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 166 (actual)
- Sponsor
- Children's Oncology Group · Network
- Sex
- All
- Age
- 50 Years
- Healthy volunteers
- Not accepted
Summary
RATIONALE: Studying samples of tissue in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors understand how well patients respond to treatment. PURPOSE: This research study is studying biomarkers in tissue samples from patients with Ewing sarcoma.
Detailed description
OBJECTIVES: * To describe the spectrum of GGAA-microsatellite polymorphisms at specific loci in genomic DNA prepared from Ewing sarcoma tumor specimens. * To determine if there are differences in GGAA-microsatellite polymorphisms in genomic DNA prepared from Ewing sarcoma tumor specimens as compared to non-afflicted European and African normal genomic DNA. * To determine if GGAA-microsatellite polymorphisms at specific loci in genomic DNA prepared from Ewing's sarcoma tumor specimens correlates with disease outcome in patients treated on COG protocol AEWS0031. * To determine whether whole-genome amplification introduces alterations in GGAA-microsatellites as compared to non-amplified genomic DNA. OUTLINE: Genomic PCR is used to amplify the microsatellites in the NR0B1 and GSTM4 promoters. In addition to determining microsatellite size, each microsatellite is sequenced following cloning into the pCR4 vector (Invitrogen) using standard topoisomerase cloning protocols.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| GENETIC | DNA analysis | |
| GENETIC | comparative genomic hybridization | |
| GENETIC | gene expression analysis | |
| GENETIC | mutation analysis | |
| GENETIC | polymerase chain reaction | |
| GENETIC | polymorphic microsatellite marker analysis | |
| OTHER | laboratory biomarker analysis |
Timeline
- Start date
- 2011-12-01
- Primary completion
- 2012-01-01
- Completion
- 2012-01-01
- First posted
- 2011-11-29
- Last updated
- 2015-05-12
Source: ClinicalTrials.gov record NCT01480518. Inclusion in this directory is not an endorsement.