NCT01454765

Generation of Haploid Stem Cells From Human Germ Cells

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 10 (estimated)
• Sponsor: Rambam Health Care Campus · Academic / Other
• Sex: Male
• Age: 18 Years – 50 Years
• Healthy volunteers: Accepted

Summary

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animals. The investigators aim at generating haploid stem cells from human germ cells that are a useful tool in genetic research and screening.

Detailed description

Human cells are diploid (contain a double set of chromosomes). The diploid genome is complex and therefore limits genetic research approaches in biomedical models. To overcome this problem experimental generation of haploid stem cells has been done in animal models. haploid cells are easier for genetic manipulations, such as knock-in and knock-out of genes, and therefore should make a better experimental model. The investigators aim at generating haploid stem cells from human haploid germ cells.

Conditions

• Induced Pluripotent Stem Cells

Interventions

Type	Name	Description
OTHER	generation of stem cells	Collected germ cells will be cultured in medium. The germ cell cultures will be infected with reprograming factors. Rare colonies with characteristic stem cell morphologies will be expanded.

Timeline

• Start date: 2011-12-01
• Primary completion: 2014-12-01
• Completion: 2015-08-01
• First posted: 2011-10-19
• Last updated: 2013-12-11

Locations

2 sites across 1 country: Israel