NCT01419509

DNA Tests in Detecting Disseminated Disease in Tumor, Blood, and Bone Marrow Samples From Patients With Rhabdomyosarcoma

DNA Methylation-based Assays for Detecting Disease Spread in Rhabdomyosarcoma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 170 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: DNA analysis of tumor, blood, and bone marrow may help doctors predict if the disease has been disseminated. PURPOSE: This research trial studies DNA tests in detecting disseminated disease in tumor, blood, and bone marrow samples from patients with rhabdomyosarcoma.

Detailed description

OBJECTIVES: * To establish a panel of hypermethylation assays for minimal disseminated disease analysis in rhabdomyosarcoma (RMS). * To apply the hypermethylation assays to a series of bone marrow specimens from RMS patients. OUTLINE: Archived tumor, bone marrow, and peripheral blood samples are analyzed for DNA hypermethylation by PCR-based assays and microarray assays. Results are then compared with each patient's clinical and pathological characteristics, and outcomes. Patterns of locus hypermethylation in tumor and paired bone marrow samples are also compared.

Conditions

• Sarcoma

Interventions

Type	Name	Description
GENETIC	DNA methylation analysis
GENETIC	microarray analysis
GENETIC	polymerase chain reaction
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2011-08-01
• Primary completion: 2016-05-01
• Completion: 2016-05-01
• First posted: 2011-08-18
• Last updated: 2016-05-17