NCT01414088

The Effects of Isotonic and Hypertonic Saline Infusion on Renal Biomarkers in Healthy Young Subjects

The Effects of Isotonic and Hypertonic Saline Infusion on Renal Biomarkers for the Measurement of Sodium and Water Channel Activity in the Nephron in Healthy Young Subjects

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 23 (actual)
• Sponsor: Regional Hospital Holstebro · Academic / Other
• Sex: All
• Age: 18 Years – 45 Years
• Healthy volunteers: Accepted

Summary

The kidneys have numerous salt and water channels and play a major role in the regulation of sodium and water. We do not know how these channels work in certain water and sodium accumulating medical conditions. The purpose of this study is to identify and measure the activity in the water and sodium channels by measuring urine biomarkers/proteins in young healthy subjects.

Detailed description

25 healthy subjects from age 18-45 are recruited. Subjects will be examined on three examination days. Four days prior to the examination days the subjects will consume a standard diet based on the amount of sodium and calories. On the examination day subjects are randomised to an infusion with either isotonic saline 23 ml/kg, hypertonic saline 7 ml/kg or glucose 23 ml/kg for a period of 50 minutes. Renal function is measured by renal clearance of 51Cr-EDTA, urinary sodium, potassium and creatinine. Urinary NCC, NKCC and ENAC will be measured to evaluate the activity of sodium channels in the nephron.

Conditions

• Nephropathy

Interventions

Type	Name	Description
OTHER	isotonic saline 0,9 mg/ml	infusion, 23 ml/kg, during 50 minutes
OTHER	hypertonic saline 2,9 mg/ml	infusion, 7 ml/kg, during 50 minutes
OTHER	glucose 5%	infusion, 23 ml/kg, during 50 minutes

Timeline

• Start date: 2011-04-01
• Primary completion: 2012-04-01
• Completion: 2012-04-01
• First posted: 2011-08-11
• Last updated: 2013-05-20

Locations

1 site across 1 country: Denmark