NCT01387724

Biomarkers in Blood and Tissue Samples From Patients With Newly Diagnosed Neuroblastoma

Neuropeptide Y and Its Receptors in Neuroblastoma

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 300 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 1 Year – 10 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of blood and tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research trial studies biomarkers in blood and tissue samples from patients with newly diagnosed neuroblastoma.

Detailed description

OBJECTIVES: * Determine the expression of neuropeptide Y (NPY) and its receptors (Rs) in human neuroblastoma (NB) tissues. * Determine whether BDNF/TrkB and TrkAIII stimulate expression of NPY and its Rs. * Determine whether NPY mediates BDNF- and TrkAIII-induced NB proliferation and survival. * Determine neurotrophins' angiogenic actions. * Identify factors released from NB cells upon NPY stimulation (proteomics). * Determine whether NPY upregulates expression of the identified proteins in NB and their Rs in endothelial cells (ECs). * Test whether inhibition of the identified pathways reduces angiogenic activity of NB-conditioned media. * Determine the mechanisms of NYP actions and signaling pathways. * Test whether blocking NPY-Y2/Y5 pathway reduces NB growth and vascularization in vivo. OUTLINE: Archived tumor tissue and serum samples are analyzed for neuropeptide Y and its receptors (Y1, Y2, and Y5) expression, neuroblastoma prognostic factors (MYCN, TrkA, TrkAIII, TrkB, BDNF, and NGF), and angiogenic markers by real-time PCR, IHC, ELISA, radioimmunoassay (RIA), mitogenic assay, caspase 3/7 activity assay, western blots, liquid chromatography, tandem mass spectrometry, proteomic assays, and other assays. Results are then analyzed and compared with patients' clinical data, including stage of disease, its phenotype, prognostic markers, age and gender, and response to treatment.

Conditions

• Neuroblastoma

Interventions

Type	Name	Description
GENETIC	RNA analysis
GENETIC	polymerase chain reaction
GENETIC	protein expression analysis
GENETIC	proteomic profiling
GENETIC	western blotting
OTHER	enzyme-linked immunosorbent assay
OTHER	immunohistochemistry staining method
OTHER	laboratory biomarker analysis
OTHER	liquid chromatography
OTHER	mass spectrometry

Timeline

• Start date: 2011-06-01
• Primary completion: 2016-05-01
• First posted: 2011-07-04
• Last updated: 2016-05-18