NCT01256801

Gene Expression Profiling of Breast Cancer Cells Predict the Response of Malignant Pleural Effusion

Gene Expression Profiling of Breast Cancer Cells in Pleural Effusion Prodict the Response of Malignant Pleural Effusion to Immunotherapy

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 36 (actual)
• Sponsor: Peking University Cancer Hospital & Institute · Academic / Other
• Sex: Female
• Age: 18 Years – 75 Years
• Healthy volunteers: Not accepted

Summary

The investigators want to develop a gene expression profile for the prediction of immunotherapy response of patients with metastatic breast cancer presenting malignant pleural effusion.

Detailed description

1. The patiets with malignant pleural effusion are randomizned to be treated with cytokins(inteleukin 2) or dendritic cells(DC) plus cytokine induced killer cells(CIK) locally. 2. Malignant pleural effusion from metastatic breast cancer patient is obtained through thoracentesis and is centrifugalized to enrich cancer cells before the therapy. 3. The enriched cancer cells are flash frozen and stored at -80℃ until processing. 4. The gene expression in pleural effusion is detected by microarray to screen gene markers that are differently expressed between groups . 5. Statistical analysis is performed using unsupervised hierarchical cluster.

Conditions

• Breast Neoplasms
• Neoplasm Metastasis
• Gene Expression Profiling
• Immunotherapy

Interventions

Type	Name	Description
BIOLOGICAL	cytokine	interleukin 2 2 million unit every week

Timeline

• Start date: 2010-11-01
• Primary completion: 2013-01-01
• Completion: 2013-12-01
• First posted: 2010-12-09
• Last updated: 2015-07-29

Locations

1 site across 1 country: China