NCT01248377

Skin Testing and ELISPOT Assay in Patients With a History of Non-Immediate Reactions to Cephalosporins

The Results of Skin Testing and The Detection of Cytokine Secretion From Drug Specific Peripheral Blood Mononuclear Cells Using ELISPOT Assay in the Diagnosis of Nonimmediate Reactions to Cephalosporins

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 28 (actual)
• Sponsor: Chulalongkorn University · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

This study aims to compare the results of skin testing and ELISPOT assay in patients with a history of non-immediate reactions to cephalosporins

Detailed description

Patients with a history of non-immediate reactions to cephalosporins will be tested by patch test and/or intradermal test. Peripheral blood mononuclear cells will be collected to analyzed cytokine secretions by ELISPOT assay

Conditions

• Cephalosporin Allergy
• Non-immediate Drug Reactions

Interventions

Type	Name	Description
PROCEDURE	Skin tests and ELISPOT assay	Patch test and/or intradermal test will be performed in patients as well as cytokine secretions from PBMCs will be analyzed by ELISPOT assay

Timeline

• Start date: 2010-06-01
• Primary completion: 2011-05-01
• Completion: 2011-05-01
• First posted: 2010-11-25
• Last updated: 2011-10-12

Locations

1 site across 1 country: Thailand