NCT01138306

Biomarkers in Samples From Young Patients With Acute Myeloid Leukemia

Implications of s-SHIP Expression and SHIP Alterations in AML

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 149 (estimated)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 21 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about biomarkers related to cancer. PURPOSE: This research study is studying biomarkers in samples from young patients with acute myeloid leukemia.

Detailed description

OBJECTIVES: * Determine the extent and prognostic implications of variable s-SHIP expression and SHIP gene mutations in pediatric patients with acute myeloid leukemia. * Determine the effects of aberrant s-SHIP expression on the PI3K/Akt pathway in these patients via electrochemiluminescence and phosphospecific flow cytometry assays. OUTLINE: RNA samples are analyzed for variable s-SHIP expression and are screened for SHIP mutations. Cryopreserved cells (with known high- or low-s-SHIP expression) are analyzed for the levels of various components of the PI3K/Akt pathway via electrochemiluminescence and phosphospecific flow cytometry.

Conditions

• Leukemia

Interventions

Type	Name	Description
GENETIC	gene expression analysis
GENETIC	mutation analysis
OTHER	flow cytometry
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2013-02-01
• Primary completion: 2016-05-01
• Completion: 2016-05-01
• First posted: 2010-06-07
• Last updated: 2016-05-18