NCT01057199

Studying Biomarkers in Cell Samples From Patients With Acute Myeloid Leukemia

Critical Role of MicroRNA-34a and MicroRNA-194 in Acute Myeloid Leukemia With CEBPA Mutations

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 6 (actual)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at biomarkers in cell samples from patients with acute myeloid leukemia.

Detailed description

OBJECTIVES: * To determine the significance of regulation of microRNA-34a and microRNA-194 by C/EBPα during granulopoiesis in patients with acute myeloid leukemia with CEBPA mutations. * To determine whether overexpression of micro RNA-34a and microRNA-194 in myeloid progenitors with C/EBPα mutations leads to granulocytic differentiation. * To examine how microRNA-34a and microRNA-194 downregulate E2F3 during granulopoiesis. * To determine the role of microRNA-34a and microRNA-194 in myeloid cell proliferation. OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including mutation analysis, gene expression analysis, RNA analysis, and fluorescence-activated cell sorting (FACS) analysis.

Conditions

• Leukemia

Interventions

Type	Name	Description
GENETIC	RNA analysis
GENETIC	gene expression analysis
GENETIC	mutation analysis
GENETIC	protein expression analysis
OTHER	fluorescence activated cell sorting
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2010-01-01
• Primary completion: 2010-06-01
• Completion: 2010-06-01
• First posted: 2010-01-27
• Last updated: 2015-05-07