NCT01054612

Tissue Sectioning by Electro-Dissociation

• Status: Withdrawn
• Phase: —
• Study type: Observational
• Enrollment: 0 (actual)
• Sponsor: University of Arkansas · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

Currently there is no technique to produce thin (0.004-0.01 mm) serial sections of large fresh tissue specimens that are suitable for high-resolution in situ protein/gene expression studies without ice artifact or fixation-induced molecular damage. Traditional frozen sectioning preserves protein and nucleic acid structure, but the inherent ice artifact precludes reconstruction of protein and mRNA expression patterns in 3-dimensions. Since the limitations of the existing sectioning techniques result from the fact that they rely on mechanical cutting which in turn require the tissue to be stiff, we suggest a new approach to cut tissue via an electro erosion process that utilizes focus radio frequency (RF).

Conditions

• Thin Sections
• Fresh Unfixed Tissues
• Fresh Unfrozen Tissues

Interventions

Type	Name	Description
OTHER	Tissue Sectioning via Electro Erosion Process	Focused RF energy can be used to produce consecutive thin sections of fresh tissue for immunohistochemical and nucleic acids analysis by electro-dissociation without thermal damage, ultimately allowing high resolution reconstruction of gene and protein expression patterns of large tissue specimens in 3D.

Timeline

• Start date: 2002-03-01
• Primary completion: 2010-01-01
• Completion: 2010-01-01
• First posted: 2010-01-22
• Last updated: 2015-03-04