Trials / Unknown
UnknownNCT01028898
Abnormal Expression Proteins, Mitochondrial DNA and miRNA of Irritable Bowel Syndrome
Screening and Identification of Abnormal Expression Proteins and miRNA From Colonic Mucosa of Patients With Irritable Bowel Syndrome.
- Status
- Unknown
- Phase
- —
- Study type
- Observational
- Enrollment
- 180 (estimated)
- Sponsor
- Chinese PLA General Hospital · Academic / Other
- Sex
- All
- Age
- 18 Years – 60 Years
- Healthy volunteers
- Accepted
Summary
In the investigators study, the investigators will focus on the screening of the related proteins and miRNA to IBS in order to reveal the possible clues or molecular mechanism for this disorder.
Detailed description
For the proteomic procedures, Our study groups extracted proteins from the cecum and sigmoid colon samples and ran on the two-dimensional gel electrophoresis (2-DE). The proteins on 2-DE were visualized by the silver stain, and then gel maps were scanned and analyzed with the Image Master 2D Elite. The obvious abnormal protein spots were identified by the mass spectrometry. Western blot 、 immunohistochemistry 、 mRNA and miRNA were also performed for further verification of the proteins.
Conditions
- Irritable Bowel Syndrome
- Digestive System Diseases
- Gastrointestinal Diseases
- Intestinal Diseases
- Colonic Diseases
- Colonic Diseases, Functional
Timeline
- Start date
- 2006-01-01
- Primary completion
- 2011-08-01
- Completion
- 2012-12-01
- First posted
- 2009-12-09
- Last updated
- 2011-02-11
Source: ClinicalTrials.gov record NCT01028898. Inclusion in this directory is not an endorsement.