NCT00918658

Study of Natural Killer Cells in Bone Marrow and Blood Samples From Patients With and Without Hematologic Cancer

Collection of Bone Marrow and Peripheral Blood for Testing of Notch-induced Natural Killer Cell Activity

• Status: Terminated
• Phase: —
• Study type: Observational
• Enrollment: 34 (actual)
• Sponsor: Case Comprehensive Cancer Center · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

RATIONALE: Collecting and storing samples of bone marrow and blood from patients with cancer to study in the laboratory may help doctors find better ways to treat the cancer. PURPOSE: This research study is looking at natural killer cells in bone marrow and blood samples from patients with hematologic cancer and from patients who do not have cancer.

Detailed description

OBJECTIVES: * To collect normal and malignant cells in bone marrow and peripheral blood samples from patients with acute myeloid leukemia, acute lymphoblastic leukemia, chronic myelogenous leukemia, or multiple myeloma and from patients with no malignancy to use as target cells in cytotoxicity assays. * To collect information about HLA type and immunophenotype of malignant cells as determined by clinical flow cytometry. OUTLINE: Bone marrow aspirates and/or peripheral blood samples are collected and used as target cells in standard cytotoxicity assays, with notch-induced natural killer (N-NK) cells as the effector cells, to determine whether N-NK cells are capable of killing malignant cells and/or non-malignant cells. Receptor-blocking antibodies may be added to determine which NK cell receptors are responsible for malignant cell recognition and killing. Information about HLA type and immunophenotype of the malignant cells is collected from the patient's medical record.

Conditions

• Leukemia
• Multiple Myeloma and Plasma Cell Neoplasm

Interventions

Type	Name	Description
OTHER	biologic sample preservation procedure	All specimens will be collected using the Pathology Residual Tissue Procurement.
OTHER	immunologic technique	Information about HLA type and immunophenotype of the malignant cells is collected from the patient's medical record.
OTHER	laboratory biomarker analysis	Bone marrow aspirates and/or peripheral blood samples are collected and used as target cells in standard cytotoxicity assays, with notch-induced natural killer (N-NK) cells as the effector cells, to determine whether N-NK cells are capable of killing malignant cells and/or non-malignant cells. Receptor-blocking antibodies may be added to determine which NK cell receptors are responsible for malignant cell recognition and killing.

Timeline

• Start date: 2008-04-01
• Primary completion: 2011-03-01
• Completion: 2011-03-01
• First posted: 2009-06-11
• Last updated: 2015-10-09

Locations

1 site across 1 country: United States