NCT00900523

Prostaglandin Metabolite in Urine Samples From Patients With Ovarian Cancer

Urine Prostaglandin-M(PGE-M) in Ovarian Cancer

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 7 (actual)
• Sponsor: Vanderbilt-Ingram Cancer Center · Academic / Other
• Sex: Female
• Age: 17 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Measuring the amount of prostaglandin metabolite in urine samples and studying tissue samples in the laboratory from patients with ovarian cancer may help doctors identify and learn more about biomarkers related to ovarian cancer and may help the study of cancer in the future. PURPOSE: This laboratory study is measuring the amount of prostaglandin metabolite in urine samples from patients with ovarian cancer.

Detailed description

OBJECTIVES: * Determine the level of prostaglandin E\_2 metabolite (PGE-M) in 24-hour urine samples collected prior to surgery or chemotherapy from patients with ovarian cancer. * Determine cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) expression by western blot and immunohistochemistry (IHC) in ovarian tumor tissue samples and matched normal ovarian tissue samples, if available, from these patients. * Correlate urine PGE-M levels with corresponding tissue COX-1 and COX-2 levels in urine and tissue samples from these patients. OUTLINE: This is a pilot study. Patients submit 24-hour urine specimens prior to surgery for evaluation of urinary levels of prostaglandin E\_2 metabolite (PGE-M). Patients also undergo assessment of tissue obtained at the time of surgery, including ovarian tumor tissue, normal surface ovarian epithelium tissue (if available), and fixed tissue embedded in a paraffin block. Tissue samples are analyzed by western blot and IHC for cyclooxygenase-1 and cyclooxygenase-2 expression.

Conditions

• Ovarian Cancer

Interventions

Type	Name	Description
GENETIC	protein expression analysis	protein expression analysis
OTHER	immunohistochemistry	immunohistochemical staining of tumor tissue to measure COX-1 and COX-2 levels

Timeline

• Start date: 2006-11-01
• Primary completion: 2007-07-01
• Completion: 2008-07-01
• First posted: 2009-05-12
• Last updated: 2013-04-18