Trials / Completed
CompletedNCT00900380
Studying Tissue Samples to Learn More About Drug Resistance in Patients With Acute Myeloid Leukemia
Comparison of P-gp Efflux and Accumulation Assay Results With ECOG 3999 Leukemia Samples
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 40 (actual)
- Sponsor
- ECOG-ACRIN Cancer Research Group · Network
- Sex
- All
- Age
- 18 Years – 120 Years
- Healthy volunteers
- Not accepted
Summary
RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about cancer and the development of drug resistance in patients. PURPOSE: This laboratory study is examining tissue samples from patients with acute myeloid leukemia to learn more about drug resistance in these patients.
Detailed description
OBJECTIVES: * Ascertain whether assessments of P-glycoprotein (P-gp) status using the accumulation assay find greater than 10% positive specimens among 30 specimens (collected from patients with acute myeloid leukemia enrolled on clinical trial ECOG-E3999) found to be negative using only the efflux assay. OUTLINE: This is a multicenter study. Cryopreserved bone marrow specimens are examined for P-glycoprotein (P-gp) by the accumulation assay and the efflux assay using DiOC\_2 dye. Flow cytometry is used for measuring activity in both assays. PROJECTED ACCRUAL: A total of 40 specimens will be accrued for this study.
Conditions
Interventions
| Type | Name | Description |
|---|---|---|
| OTHER | flow cytometry | |
| OTHER | immunological diagnostic method | |
| OTHER | laboratory biomarker analysis |
Timeline
- Start date
- 2006-03-28
- Primary completion
- 2013-12-30
- Completion
- 2013-12-30
- First posted
- 2009-05-12
- Last updated
- 2017-05-19
Source: ClinicalTrials.gov record NCT00900380. Inclusion in this directory is not an endorsement.