NCT00900341

Studying Tumor Tissue Samples From Patients With Soft Tissue Sarcoma

LABORATORY STUDIES OF SOFT TISSUE SARCOMAS

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 27 (actual)
• Sponsor: University of Arizona · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at tumor tissue samples from patients with soft tissue sarcoma.

Detailed description

OBJECTIVES: * To develop a pilot program for the collection, archiving, and experimental analyses of soft tissue sarcomas (STS). * To obtain STS tumors from cancer patients undergoing tumor removal or core needle biopsies. * To evaluate cellular and molecular factors involved in STS oncogenesis by studying samples derived from human tumor specimens. * To develop an accompanying bioinformatics database of the demographics of STS patients from which tumor specimens are derived for experimental study. OUTLINE: Tumor tissue samples are analyzed for gene and protein expression by cDNA microarray analysis, RT-PCR, immunohistochemistry, in vitro transformation assays, RNA interference, and Western blot or immunoprecipitation.

Conditions

• Sarcoma

Interventions

Type	Name	Description
GENETIC	DNA analysis
GENETIC	gene expression analysis
GENETIC	protein expression analysis
GENETIC	reverse transcriptase-polymerase chain reaction
GENETIC	western blotting
OTHER	immunohistochemistry staining method
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2005-08-01
• Primary completion: 2013-06-01
• Completion: 2013-06-01
• First posted: 2009-05-12
• Last updated: 2014-03-21

Locations

1 site across 1 country: United States