NCT00900328

Study of Tumor Samples From Patients With Lung Cancer

A Pilot Project to Study the Expression of c-MET and p53 in Resected Lung Adenocarcinoma Specimens

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 280 (actual)
• Sponsor: Alliance for Clinical Trials in Oncology · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at tumor samples from patients with lung cancer.

Detailed description

OBJECTIVES: Primary * To determine the correlation between c-Met expression, mutation and amplification, with stage and overall survival in patients with adenocarcinoma (AC) of the lung. Secondary * To determine the correlation with epithelial mesenchymal transition (EMT), EGFR mutations and expression, Kras mutations, p53 mutations, c-CBL protein expression, mutation, loss of heterozygosity (LOH), DUB3 expression \& regulation, and ALK translocation, with respect to survival. * To determine the correlation with circulating c-Met and HGF in AC and evaluate prognostic implications of circulating markers in AC of lung. * To determine (when available) levels of circulating Met and HGF in serum before and after surgery. OUTLINE: This is a multicenter study. Previously collected tissue samples from patients enrolled in CALGB 140202 are assessed for mutation analysis of c-Met, EGFR, and K-ras. DNA is examined by PCR, followed by agarose gel electrophoresis; gene amplification of c-Met is examined by real time quantitative PCR; met/HF protein in serum is examined by ELISA; and c-Met, EGFR, p53, c-CBL, DUB3 enzyme, and ALK, and epithelial mesenchymal transition examined by IHC.

Conditions

• Lung Cancer

Interventions

Type	Name	Description
OTHER	laboratory biomarker analysis	Correlative Studies

Timeline

• Start date: 2008-09-01
• Primary completion: 2012-02-01
• Completion: 2012-09-01
• First posted: 2009-05-12
• Last updated: 2017-08-08

Locations

1 site across 1 country: United States