NCT00899743

S9333A Study of Blood and Bone Marrow Samples From Patients With Previously Untreated Primary Acute Myeloid Leukemia

Determining the Clinicopathological Significance of CaMKIIgamma Activation in AML

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 132 (actual)
• Sponsor: SWOG Cancer Research Network · Network
• Sex: All
• Age: 56 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of blood and bone marrow from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This research study is looking at blood and bone marrow samples from patients with previously untreated primary acute myeloid leukemia.

Detailed description

OBJECTIVES: * Determine whether calmodulin-dependent protein kinase II-γ (CaMKIIγ) activation (autophosphorylation) is associated with specific clinicopathological variables and outcomes in blood and bone marrow samples from patients with previously untreated primary acute myeloid leukemia. OUTLINE: Blood and bone marrow samples are analyzed by western blot using SDS-PAGE and immunoblotting using anti-calmodulin-dependent protein kinase II-γ (CaMKIIγ) and anti-phosphospecific (Thr286, 287) CaMKIIγ antibodies with signals detected via chemiluminescence. CaMKIIγ activation levels are linked to clinical variables within the SWOG database.

Conditions

• Leukemia

Interventions

Type	Name	Description
GENETIC	polyacrylamide gel electrophoresis	polyacrylamide gel electrophoresis
GENETIC	western blotting	western blotting
OTHER	immunologic technique	immunologic technique
OTHER	laboratory biomarker analysis	laboratory biomarker analysis

Timeline

• Start date: 2008-08-01
• Primary completion: 2009-06-01
• Completion: 2009-06-01
• First posted: 2009-05-12
• Last updated: 2015-03-06