NCT00899652

DNA Analysis of Bone Marrow and Blood Samples From Young Patients With Acute Myeloid Leukemia or Acute Lymphoblastic Leukemia

The Molecular Epidemiology of de Novo and Treatment Related 11q23 Leukemia in the Young

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 149 (actual)
• Sponsor: Children's Oncology Group · Network
• Sex: All
• Age: 21 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of bone marrow and blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at DNA in bone marrow and blood samples from young patients with acute myeloid leukemia or acute lymphoblastic leukemia.

Detailed description

OBJECTIVES: * Characterize the clinical, morphologic, immunologic, cytogenetic, and molecular heterogeneity of acute lymphoblastic leukemia or acute myeloid leukemia (AML) in infants and monoblastic variants of AML in young patients. * Characterize the clinical, morphologic, immunologic, cytogenetic, and molecular heterogeneity of secondary AML in young patients. * Compare secondary AML vs de novo AML at the level of Southern blot, breakpoint sequence, and DNA topoisomerase II cleavage sites. OUTLINE: This is a multicenter study. Bone marrow or blood are collected and analyzed by Southern blot for chromosome 11q23 breakpoints and translocations. Samples from patients with secondary acute myeloid leukemia are also examined for MLL gene rearrangements. PROJECTED ACCRUAL: A total of 250 patients will be accrued for this study.

Conditions

• Leukemia

Interventions

Type	Name	Description
GENETIC	Southern blotting
GENETIC	chromosomal translocation analysis
GENETIC	cytogenetic analysis
GENETIC	gene rearrangement analysis
GENETIC	mutation analysis

Timeline

• Start date: 1997-01-01
• Primary completion: 2005-09-01
• Completion: 2006-09-01
• First posted: 2009-05-12
• Last updated: 2017-04-26