NCT00899366

DNA Analysis of Tissue From Patients With T-Cell Acute Lymphoblastic Leukemia

Molecular Mechanisms of NOTCH Induced Transformation in T-ALL

• Status: Completed
• Phase: —
• Study type: Observational
• Enrollment: 49 (actual)
• Sponsor: ECOG-ACRIN Cancer Research Group · Network
• Sex: All
• Age: 18 Years – 120 Years
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at tissue samples from patients with T-cell acute lymphoblastic leukemia.

Detailed description

OBJECTIVES: * Identify the transcriptional signatures associated with the presence of NOTCH1 mutations in primary T-cell acute lymphoblastic leukemia (T-ALL) cells using oligonucleotide microarrays. * Characterize the global changes on gene expression resulting from the inactivation of NOTCH signaling in human T-ALL lymphoblasts. OUTLINE: This is a multicenter study. Frozen lymphoblast samples from patients with T-cell acute lymphoblastic leukemia (NOTCH1-mutated and wild type) are assessed for genetic expression profiles and mutations by microarray analysis and activated NOTCH1 protein by western blot analysis. PROJECTED ACCRUAL: A total of 48 samples from patients will be accrued for this study.

Conditions

• Leukemia

Interventions

Type	Name	Description
GENETIC	microarray analysis
GENETIC	mutation analysis
OTHER	immunologic technique

Timeline

• Start date: 2006-12-28
• Primary completion: 2009-07-30
• Completion: 2009-07-30
• First posted: 2009-05-12
• Last updated: 2020-02-05