NCT00899327

Biomarkers in Patients at Risk of Developing Myelodysplastic Syndrome or Other Disorders and in Healthy Participants

Molecular and Functional Characterisation of Bone Marrow Function in Normal Subjects, Myelodysplastic Syndromes (MDS) and Secondary Disorders of Heamatopoiesis

• Status: Unknown
• Phase: —
• Study type: Observational
• Enrollment: 200 (estimated)
• Sponsor: Oxford University Hospitals NHS Trust · Academic / Other
• Sex: All
• Age: 18 Years
• Healthy volunteers: Accepted

Summary

RATIONALE: Studying samples of blood and bone marrow in the laboratory from patients at risk of developing myelodysplastic syndrome may help doctors learn more about changes that occur in DNA and identify biomarkers related to disorders of the blood and bone marrow. PURPOSE: This research study is looking at biomarkers in patients at risk of developing myelodysplastic syndrome or other disorders and in healthy participants.

Detailed description

OBJECTIVES: Primary * To identify novel biomarkers of disease that would help in the initial diagnosis of myelodysplastic syndromes (MDS). Secondary * To understand the genesis of anemia in cancers. * To identify novel biomarkers of disease that predict progression of MDS to acute myeloid leukemia. OUTLINE: Blood and bone marrow samples are collected. Hemopoietic stem cells (HSC) and progenitor cells are isolated from samples for analysis. Some of these HSC and progenitor cells are used for functional assays. From the rest of the cells, DNA, RNA, and protein is extracted for molecular analyses, including gene mutation analysis, gene methylation assays, chromatin immunoprecipitation, microarray, and real-time polymerase chain reaction.

Conditions

• Leukemia
• Myelodysplastic Syndromes

Interventions

Type	Name	Description
GENETIC	DNA methylation analysis
GENETIC	RNA analysis
GENETIC	cytogenetic analysis
GENETIC	gene expression analysis
GENETIC	mutation analysis
GENETIC	polymerase chain reaction
GENETIC	protein expression analysis
OTHER	laboratory biomarker analysis

Timeline

• Start date: 2007-01-01
• Primary completion: 2011-12-01
• First posted: 2009-05-12
• Last updated: 2009-07-08

Locations

1 site across 1 country: United Kingdom