NCT00899002

Biomarkers in Stored Tumor Samples From Younger Patients With Liver Cancer

Molecular Analysis of Liver Cancer

• Status: Withdrawn
• Phase: —
• Study type: Observational
• Enrollment: 0 (actual)
• Sponsor: Vanderbilt-Ingram Cancer Center · Academic / Other
• Sex: All
• Age: —
• Healthy volunteers: Not accepted

Summary

RATIONALE: Studying samples of tumor tissue from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. PURPOSE: This laboratory study is looking at biomarkers in stored tumor samples from younger patients with liver cancer.

Detailed description

OBJECTIVES: * To characterize, at a molecular level, archived samples of tissue from young patients with fibrolamellar carcinoma and hepatocellular carcinoma in non-cirrhotic livers matched for age and sex. * To perform genomic analysis on these tissue samples using array comparative genomic hybridization. * To perform targeted gene mutation analysis on these samples by PCR. * To perform proteomic profiling on fixed tissues in these samples by various proteomic methods, including IHC and mass spectrometry. * To look for association between molecular aberrations and clinicopathologic features in these samples. OUTLINE: Archived tissue samples are collected from the pathology department at Vanderbilt University Medical Center and from the Mayo Clinic in Rochester, Minnesota. Tissue samples are analyzed by genomic analysis using array comparative genomic hybridization, target gene mutation analysis by PCR, and proteomic profiling on fixed tissues using various proteomic methods, including IHC and mass spectrometry. Samples are also examined for association between molecular aberrations and clinicopathologic features found in each disease. Clinical patient data (i.e., age, sex, race, date of diagnosis, risk factors, histology, surgical staging, follow-ups, date of death, and adjuvant therapy) are also collected.

Conditions

• Liver Cancer

Interventions

Type	Name	Description
GENETIC	comparative genomic hybridization
GENETIC	molecular genetic technique
GENETIC	mutation analysis
GENETIC	polymerase chain reaction	Not noted
GENETIC	proteomic profiling
OTHER	immunohistochemistry staining method	not noted
OTHER	laboratory biomarker analysis	not noted
OTHER	mass spectrometry	not noted
OTHER	medical chart review	not noted

Timeline

• Start date: 2007-07-01
• Primary completion: 2009-05-01
• Completion: 2009-05-01
• First posted: 2009-05-12
• Last updated: 2013-04-10