Trials / Completed
CompletedNCT00898391
Study of DNA in Blood Samples From Patients With Neuroblastoma
Analysis of Circulating MYCN DNA in Serum Samples Obtained From Patients With Neuroblastoma
- Status
- Completed
- Phase
- —
- Study type
- Observational
- Enrollment
- 329 (actual)
- Sponsor
- Children's Oncology Group · Network
- Sex
- All
- Age
- —
- Healthy volunteers
- Not accepted
Summary
This research trial studies is deoxyribonucleic acid (DNA) in blood samples from patients with neuroblastoma. Studying samples of blood from patients with cancer in the laboratory may help doctors learn more about changes that occur in DNA and identify biomarkers related to cancer. It may also help doctors predict how patients will respond to treatment.
Detailed description
OBJECTIVES: I. Perform in a blind study a multicentric study by analyzing serum from patients at diagnosis (with a known tumor v-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog (MYCN) status determined in the Children's Oncology Group \[COG\] reference lab), in order to confirm our previous data and to define the most appropriate MYCN/control ratio cutoff capable of determining positive and negative cases. II. Define the limits of the procedure in non-metastatic patients and in those with reduced tumor burden (International Neuroblastoma Staging System \[INSS\] stage 1 and 2). OUTLINE: Circulating DNA is extracted from serum. Polymerase chain reaction (PCR) amplification of MYCN is performed and analyzed by agarose gel electrophoresis. Real-time quantitative PCR is also performed.
Conditions
- Disseminated Neuroblastoma
- Localized Resectable Neuroblastoma
- Localized Unresectable Neuroblastoma
- Recurrent Neuroblastoma
- Regional Neuroblastoma
- Stage 4S Neuroblastoma
Interventions
| Type | Name | Description |
|---|---|---|
| OTHER | laboratory biomarker analysis | Correlative studies |
| GENETIC | polymerase chain reaction | Correlative studies |
| GENETIC | polyacrylamide gel electrophoresis | Correlative studies |
| GENETIC | DNA analysis | Correlative studies |
Timeline
- Start date
- 2007-06-01
- Primary completion
- 2016-05-01
- Completion
- 2016-05-01
- First posted
- 2009-05-12
- Last updated
- 2016-05-18
Locations
1 site across 1 country: United States
Source: ClinicalTrials.gov record NCT00898391. Inclusion in this directory is not an endorsement.