NCT00858273

Glutathione Metabolism in Adolescents With Type 1 Diabetes - Study B

Regulation of Glutathione Homeostasis in Adolescents With Type 1 Diabetes - Study B

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 41 (actual)
• Sponsor: Nemours Children's Clinic · Academic / Other
• Sex: All
• Age: 12 Years – 21 Years
• Healthy volunteers: Not accepted

Summary

Glutathione is normally present at high levels in the blood and plays an important role in the body's defense against oxidative stress, that is, against the damage caused to the body by several reactive oxygen species produced by the metabolism of most nutrients, including glucose. Glutathione is a small peptide made from 3 amino acids, glutamate, cysteine, and glycine. This study is looking at how blood sugar levels may affect the way glutathione is made and used by the body. Since glutathione is continuously synthesized and broken down, the amount of glutathione present in the blood depends on the balance between its rate of synthesis and its rate of use. In earlier studies, we found that in poorly controlled diabetic teenagers, glutathione was low, not because it was not produced fast enough, but because it was used at an excessive rate. In this study, we want to find out whether improving blood sugar control will increase glutathione levels, and, if so, how long this will take. We also hope to find out if oral supplementation with a mixture of several antioxidant vitamins and minerals will increase glutathione levels more than taking a placebo.

Detailed description

Forty adolescents with T1D will undergo a measurement of blood glutathione concentration and markers of oxidative stress (plasma protein-bound 3-nitrotyrosine, and urinary 8OH-2-dG, and F2-isoprostane excretion, markers of oxidative damage to protein, DNA and lipids, respectively) while at near normoglycemia, on two separate occasions: * first, when in poor glucose control (HbA1c\>7.5%); and * secondly, after 3 to 9 months months of improved blood glucose control, along with the administration of either a placebo, or a mixture of antioxidant minerals and vitamins based on a randomization scheme. Between the two metabolic study days, patients will receive the same intensified diabetes regimen consisting of education and counseling, home blood glucose monitoring, multiple daily insulin injections (MDI), diet plan, and frequent phone contact with a certified diabetes educator.

Conditions

• Diabetes Mellitus, Type 1

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	Antioxidant supplement	1 capsule daily with dinner
OTHER	Diabetes treatment	Intensification of diabetes treatment regimen, including education and counseling, home blood glucose monitoring, multiple daily insulin injections (MDI), diet plan, and frequent phone contact with a certified diabetes educator
DRUG	Regular Insulin	Regular Insulin, IV, to maintain blood glucose in normoglycemic range (70-140) during metabolic study

Timeline

• Start date: 2008-03-01
• Primary completion: 2011-03-01
• Completion: 2011-08-01
• First posted: 2009-03-09
• Last updated: 2023-05-17
• Results posted: 2023-05-17

Locations

1 site across 1 country: United States