NCT00777387

Randomized Controlled Trial Comparing Slow-freezing to Vitrification of Oocytes

Cryopreservation of Oocytes: A Randomized Controlled Trial Comparing Slow-freezing to Vitrification

• Status: Terminated
• Phase: N/A
• Study type: Interventional
• Enrollment: 14 (actual)
• Sponsor: Samuel Lunenfeld Research Institute, Mount Sinai Hospital · Academic / Other
• Sex: Female
• Age: 18 Years – 37 Years
• Healthy volunteers: Accepted

Summary

Preservation of fertility is limited. Current methods include embryo cryopreservation and while still experimental, ovarian cryopreservation. In single women who are at risk for infertility secondary to cancer or couples who have ethical reasons to avoid embryo cryopreservation, oocyte cryopreservation is an alternative Purpose of study is to compare slow freeze to vitrification of human oocytes. Successful cryopreservation and subsequent thawing programs are characterized by avoiding ice crystal formation.Vitrification, newer alternative to slow freeze method, process by which water is prevented from forming ice. Our hypothesis is that higher survival rate per oocyte is to be expected in the vitrification group.

Detailed description

Although several reviews of smaller studies of cryoprotectant methodology exist randomized controlled trials,comparing slow-freeze-rapid-thaw procedure to vitrification of oocytes, are not available. The purpose of this study is to compare these two methods of oocyte cryopreservation in human oocytes.

Conditions

• Infertility

Interventions

Type	Name	Description
PROCEDURE	slow-freeze	Slow-freeze-rapid-thaw procedure of oocytes is characterized by prolonged exposure of the cell to cryoprotectants in a low metabolic state at very low temperatures
PROCEDURE	vitrification	Vitrification is the process by which water is prevented from forming ice due to the viscosity of a highly concentrated cryoprotectant cooled at an extremely rapid rate

Timeline

• Start date: 2008-08-01
• Primary completion: 2014-01-01
• Completion: 2014-01-01
• First posted: 2008-10-22
• Last updated: 2014-01-07

Locations

1 site across 1 country: Canada