NCT00680355

Bioavailability of Golden Rice Carotenoids in Humans

• Status: Completed
• Phase: N/A
• Study type: Interventional
• Enrollment: 14 (actual)
• Sponsor: Tufts University · Academic / Other
• Sex: All
• Age: 40 Years – 70 Years
• Healthy volunteers: Accepted

Summary

This investigation protocol uses an isotope reference method to evaluate bioavailability of ß-C in rice and its bioconversion to vitamin A. A well-nourished population will be fed hydroponically grown Golden Rice containing \~ 1 mg of β-carotene at day 8 after a reference dose of vitamin A at day 1. The blood samples will be collected up to 33 days after the doses. The absorption kinetics will be determined by tracking both vitamin A and β-carotene in human serum. Blood responses to Golden Rice β-carotene will be determined and evaluated. The vitamin A value of Golden Rice can thus be quantitatively determined.

Detailed description

Recently, scientists have genetically engineered "golden rice" that contains 1.6 ug ß-carotene in a gram of dry rice (12). Since the vitamin A equivalency varies from 2 ug (oil dose) to 27 ug (vegetable) carotene to 1 retinol equivalent and the equivalency is matrix dependent, it is necessary to know what the vitamin A equivalency of golden rice beta-carotene is. The determination of the absorption and conversion of golden rice beta-carotene is of importance for designing food programs in many rice-eating regions of the world, where vitamin A deficiency is common. To evaluate the absorption and intestinal conversion of rice beta-carotene after an acute dose of golden rice, an intrinsically labeled Golden Rice with a labeled reference dose of vitamin A are used. We propose to conduct a pilot study on the US adults with normal vitamin A status (n = 15). The specific aims of this pilot study are: 1. To determine ß-carotene response kinetics following a meal of golden rice with 0, 5, or 10 g fat (butter); 2. To determine retinol kinetics from the golden rice and from the labeled vitamin A dose; 3. To determine vitamin A value of golden rice ß-carotene in adults with normal vitamin A status. The determination of the absorption and conversion of golden rice ß-carotene is of importance for designing food programs in many rice-eating regions of the world, where vitamin A deficiency is common.

Conditions

• Healthy

Interventions

Type	Name	Description
DIETARY_SUPPLEMENT	golden rice	An acute dose of cooked golden rice containing \~ 1 mg of ß-carotene
DIETARY_SUPPLEMENT	corn oil 0g	0 g of corn oil in the golden rice meal
DIETARY_SUPPLEMENT	corn oil 10g	10g corn oil in the golden rice meal
DIETARY_SUPPLEMENT	coren oil 5 g	golden rice meal with 5 g corn oil

Timeline

• Start date: 2004-08-01
• Primary completion: 2012-08-01
• Completion: 2012-08-01
• First posted: 2008-05-20
• Last updated: 2013-07-01

Locations

1 site across 1 country: United States